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polyethylenimine hcl max, linear  (Kyfora Bio)


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    Kyfora Bio polyethylenimine hcl max, linear
    Polyethylenimine Hcl Max, Linear, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 95/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/linear+polyethylenimine/Polyethylenimine+HCl+MAX%2C+Linear/custom%4024885-2%4042427034
    Average 95 stars, based on 76 article reviews
    polyethylenimine hcl max, linear - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Transfection:

    Article Title: Genetically encoded assembly recorder temporally resolves cellular history
    Article Snippet: .. When cells reached 50–70% confluence, genes were delivered using 25 K linear polyethylenimine (Polysciences) following the protocol previously reported or Lipofectamine 3000 transfection kit (Thermo). ..

    Article Title: Selective Inhibition of Integrin β3 Topology Provides a Safer Antithrombotic Strategy.
    Article Snippet: .. For antibody purification, FreeStyle 293-F cells (Thermo Fisher Scientific) were transfected with plasmids encoding the heavy and light chains of each clone using linear polyethylenimine (Polysciences). .. The cells were cultured in FreeStyle 293 expression medium (Thermo Fisher Scientific) at 37◦C in suspension, supplemented with 0.5% tryptone (Sigma) 24 h post-transfection.

    Article Title: Large Library Docking for Polypharmacology
    Article Snippet: .. In brief, HEK293T cells (gift from the Chair of Physiology, FAU Erlangen-Nürnberg) were transfected with 200 ng receptor plasmid (receptor:G α :G β :Gγ ratio 2:0.5:1:4) using linear polyethylenimine (PEI, Polysciences, 3:1 PEI:DNA ratio). .. The DNA was complemented to a total amount of 1 μg DNA per 3 · 10 5 cells with ssDNA (Sigma-Aldrich) and 10,000 cells per well were transferred into 96-well half a rea plates (Greiner, Frickenhausen, Germany).

    Article Title: Functional protein design and enhancement with ontology reinforcement iteration.
    Article Snippet: .. Transfection was performed using Linear Polyethylenimine (PEI, MW 25,000, Polysciences, Cat# 23966) with a DNA:PEI mass ratio of 1:3. ..

    Article Title: Kindlin-2-Moesin interaction orchestrates sprouting angiogenesis via modulating endothelial membrane mechanics and VEGF signaling
    Article Snippet: .. Transfection was performed using linear polyethylenimine (Polysciences). ..

    Article Title: Large Library Docking for Polypharmacology.
    Article Snippet: .. In brief, HEK293T cells (gift from the Chair of Physiology, FAU Erlangen-Nürnberg) were transfected with 200 ng receptor plasmid (receptor:Gα:Gβ:Gγ ratio 2:0.5:1:4) using linear polyethylenimine (PEI, Polysciences, 3:1 PEI:DNA ratio). .. The DNA was complemented to a total amount of 1 μg DNA per 3 · 105 cells with ssDNA (Sigma-Aldrich) and 10,000 cells per well were transferred into 96-well half a rea plates (Greiner, Frickenhausen, Germany).

    Produced:

    Article Title: Proteomic profiling of ANO10-deficient SH-SY5Y cells reveals candidate pathways relevant to SCAR10.
    Article Snippet: .. Lentiviral vectors were produced by cotransfecting HEK293 cells with 5 μg of pMD2.VSVG, 15 μg of pCMVΔ8.74, and 20 μg of pLKO.1-shANO10 (Erasmus MC, Rotterdam, The Netherlands) diluted in 500 μL of Opti-MEM, using linear polyethylenimine (Polysciences, Warrington, PA, USA). .. A pLKO.1-scramble shRNA vector (Erasmus Center for Biomics) and the PGK-GFP vector 69 served as controls.

    Article Title: Rational design of synthetic proteins using a genome-scale CRISPR screen
    Article Snippet: All cell lines were maintained at 37 °C and 5% CO 2 with regular passaging and regular mycoplasma testing using the MycoAlert Plus Mycoplasma Detection Kit (Lonza LT07-701). .. Lentivirus was produced by transfecting HEK293FT producer cells plated in 6-well dishes with 1 μg of a transfer plasmid encoding the desired expression vector alongside 0.8 μg of the packaging plasmid psPAX2 (Addgene 12260) and 0.55 μg of the envelope plasmid pMD2.G (Addgene 12259), using 5.5 μL linear polyethylenimine (PEI, MW 25,000; Polysciences 23966). .. Transfer plasmids included EGFP-d2PEST-2A-Hygro plasmid (Addgene 138152) to generate GFP expression lines, dCas9_VP64-Blast (Addgene 61425) for K562-GFP-CRISPRa, lentiCas9-Blast (Addgene 52962) for A549-Cas9 and HEK293FT-Cas9 lines, and others cloned for this study (see Plasmid design and cloning ).

    Antibody Purification:

    Article Title: Selective Inhibition of Integrin β3 Topology Provides a Safer Antithrombotic Strategy.
    Article Snippet: .. For antibody purification, FreeStyle 293-F cells (Thermo Fisher Scientific) were transfected with plasmids encoding the heavy and light chains of each clone using linear polyethylenimine (Polysciences). .. The cells were cultured in FreeStyle 293 expression medium (Thermo Fisher Scientific) at 37◦C in suspension, supplemented with 0.5% tryptone (Sigma) 24 h post-transfection.

    Plasmid Preparation:

    Article Title: Large Library Docking for Polypharmacology
    Article Snippet: .. In brief, HEK293T cells (gift from the Chair of Physiology, FAU Erlangen-Nürnberg) were transfected with 200 ng receptor plasmid (receptor:G α :G β :Gγ ratio 2:0.5:1:4) using linear polyethylenimine (PEI, Polysciences, 3:1 PEI:DNA ratio). .. The DNA was complemented to a total amount of 1 μg DNA per 3 · 10 5 cells with ssDNA (Sigma-Aldrich) and 10,000 cells per well were transferred into 96-well half a rea plates (Greiner, Frickenhausen, Germany).

    Article Title: Rational design of synthetic proteins using a genome-scale CRISPR screen
    Article Snippet: All cell lines were maintained at 37 °C and 5% CO 2 with regular passaging and regular mycoplasma testing using the MycoAlert Plus Mycoplasma Detection Kit (Lonza LT07-701). .. Lentivirus was produced by transfecting HEK293FT producer cells plated in 6-well dishes with 1 μg of a transfer plasmid encoding the desired expression vector alongside 0.8 μg of the packaging plasmid psPAX2 (Addgene 12260) and 0.55 μg of the envelope plasmid pMD2.G (Addgene 12259), using 5.5 μL linear polyethylenimine (PEI, MW 25,000; Polysciences 23966). .. Transfer plasmids included EGFP-d2PEST-2A-Hygro plasmid (Addgene 138152) to generate GFP expression lines, dCas9_VP64-Blast (Addgene 61425) for K562-GFP-CRISPRa, lentiCas9-Blast (Addgene 52962) for A549-Cas9 and HEK293FT-Cas9 lines, and others cloned for this study (see Plasmid design and cloning ).

    Article Title: Large Library Docking for Polypharmacology.
    Article Snippet: .. In brief, HEK293T cells (gift from the Chair of Physiology, FAU Erlangen-Nürnberg) were transfected with 200 ng receptor plasmid (receptor:Gα:Gβ:Gγ ratio 2:0.5:1:4) using linear polyethylenimine (PEI, Polysciences, 3:1 PEI:DNA ratio). .. The DNA was complemented to a total amount of 1 μg DNA per 3 · 105 cells with ssDNA (Sigma-Aldrich) and 10,000 cells per well were transferred into 96-well half a rea plates (Greiner, Frickenhausen, Germany).

    Expressing:

    Article Title: Rational design of synthetic proteins using a genome-scale CRISPR screen
    Article Snippet: All cell lines were maintained at 37 °C and 5% CO 2 with regular passaging and regular mycoplasma testing using the MycoAlert Plus Mycoplasma Detection Kit (Lonza LT07-701). .. Lentivirus was produced by transfecting HEK293FT producer cells plated in 6-well dishes with 1 μg of a transfer plasmid encoding the desired expression vector alongside 0.8 μg of the packaging plasmid psPAX2 (Addgene 12260) and 0.55 μg of the envelope plasmid pMD2.G (Addgene 12259), using 5.5 μL linear polyethylenimine (PEI, MW 25,000; Polysciences 23966). .. Transfer plasmids included EGFP-d2PEST-2A-Hygro plasmid (Addgene 138152) to generate GFP expression lines, dCas9_VP64-Blast (Addgene 61425) for K562-GFP-CRISPRa, lentiCas9-Blast (Addgene 52962) for A549-Cas9 and HEK293FT-Cas9 lines, and others cloned for this study (see Plasmid design and cloning ).



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